FIGURE 1.

Fluorescent proteins fused to RecA partially suppress the UV sensitivity and SOS induction defects of a recA⁻ strain. A, fresh log-phase cultures were diluted, plated onto L plates, and irradiated at various doses of UV light. Open squares, AB1157 (wild-type); open circles, BIK733 (ΔrecA) with the pBR322 empty vector; closed circles, BIK733 with pNG1 (recA803-mrfp1); closed triangles, BIK733 with pSJS1379 (recA-gfp). B, cells were assayed (in duplicate) for β-galactosidase activity originating from a fusion to the sulA gene at the indicated times. The isogenic strains are derivatives of DM4000 (39); open squares, DM4000 (recA⁺); open circles, SS2060 (Δ(recA)::kan); and closed circles, SS2065 (recA803-mrfp1). The data are the mean ± S.E. of at least three independent experiments.