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Stopped flow kinetic measurements of iron oxidation after addition of Fe2+ to apo-BFR at 25 °C. A, Fe2+ was added to final concentrations of 0, 5, 10, 15, 20, 25, 30, 40, and 50 μm to subunit dimer (6 μm BFR) in 0.1 m MES buffer (pH 6.5). B, final absorbance change after 30 s of iron oxidation at various ratios of Fe2+/BFR subunit concentration.
