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Kinetic measurements of iron oxidation after addition of Fe2+ to apo-BFR (6 μm) (25 °C). Fe2+ was added to final concentrations of 12, 24, and 36 μm to 6 μm (A) BFR subunit dimer, (B) subunit dimer E47Q, (C) subunit dimer D50N, (D) subunit dimer D126N, and (E) subunit dimer E47Q/D50N/D126N in 0.1 m MES buffer (pH 6.5). F, auto-oxidation of Fe2+ was measured in 0.1 m MES buffer (pH 6.5) in the absence of protein.
