FIGURE 6.

Binding characteristics of r0843. A, fresh human parotid saliva was subjected to SDS-PAGE, transferred to membrane, and probed with r0843 or the gp340-specific monoclonal antibody mAb 213-6 in the presence or absence of 1 mm CaCl₂. Binding was detected with S-protein-HRP or with HRP-conjugated anti-mouse antibody. Control, staining with S-protein-HRP only. B, the binding of r0843 to gp340 and other glycoproteins in an ELISA in the presence (gray bars) and absence (black bars) of 1 mm calcium. C, different amounts (1, 4, 20, and 40 μg/ml from bottom to top) of r0843 were allowed to bind to the gp340-coated surface in a surface plasmon resonance assay. D, binding of r0843 (40 μg/ml) at different CaCl₂ concentrations (0, 0.01, 0.02, 0.05, 0.5, and 1.0 mm from bottom to top) to the gp340-coated surface. RU, resonance units.