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. 2009 May 13;284(28):18824–18832. doi: 10.1074/jbc.M109.013243

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — ERα and ErbB4 protein immunoprecipitation. HEK293 (A and B), HeLa (C and D), and CHOK1 cells (E and F) were transfected with V5-tagged WT-ERα or Δ7-ERα for 48 h. Protein from cells was then harvested and immunoprecipitated with ErbB4 antibody. The immunoprecipitants were separated by SDS-PAGE and immunoprobed with V5 antibody. Immunoblotting for V5 in whole cell lysates (A, C, and E) and immunoblotting for V5 after pulldown with ErbB4 c-terminal antibody (B, D, and F) are shown. Whole cell lysates and immunoprecipitated bands (V5-WT-ERα, ∼69 kDa; V5-Δ7-ERα, ∼48 kDa) are shown. NS, nonspecific.