TABLE 1.

Number of SteT F-D curves analyzed for each pulling velocity and substrate condition

Pulling velocity	Number of analyzed F-D curves
	Substrate-free	5 mml-serine	5 mml-threonine
nm/s
145	97	79	49
311	51	73	65
654	132	128	127
1090	101	86	68
2180	116	85	90
2910	106	109	86
4360	71	90	84
8720	71	81	109