TABLE 2.

Contour lengths and rupture forces of interactions stabilizing structural segments of SteT

Contour lengths represent most probable peak positions (measured in amino acids) obtained from Gaussian fits to the contour length distributions (Fig. 1D). Forces represent most probable forces obtained from Gaussian fits to the experimental force distributions (supplemental Figs. S1–S3). Data were recorded at a pulling velocity of 654 nm/s.

Substrate-free SteT		l-Serine		l-Threonine
Contour length ± S.D.	Most probable force ± S.D.	Contour length ± S.D.	Most probable force ± S.D.	Contour length ± S.D.	Most probable force ± S.D.
aa	pN	aa	pN	aa	pN
70.2 ± 7.7	80 ± 18	69.4 ± 7.4	76 ± 18	70.2 ± 5.0	84 ± 18
79.2 ± 1.1	92 ± 30	79.4 ± 1.2	107 ± 31	79.9 ± 1.1	112 ± 24
146.8 ± 5.0	51 ± 15	147.3 ± 5.9	55 ± 15	146.8 ± 6.2	63 ± 16
191.2 ± 3.1	50 ± 20	192.1 ± 5.4	51 ± 21	193.1 ± 7.5	62 ± 27
237.0 ± 4.6	49 ± 13	237.6 ± 6.1	58 ± 17	235.4 ± 9.4	56 ± 24
303.9 ± 6.0	55 ± 16	301.8 ± 5.1	60 ± 14	299.5 ± 7.9	66 ± 18
424.9 ± 9.7	70 ± 18	422.5 ± 7.6	77 ± 12	418.7 ± 9.9	79 ± 14