Fig. 1.

Reverse association of laminin-binding glycan expression with malignancy of human prostate and breast carcinoma cells. (A) FACS analysis showed that invasive human prostate (DU-145, LNCaP, and PC-3) and breast (MCF7, MDA-MB-231, and MDA-MB-435) carcinoma cells showed reduced expression of laminin-binding glycans on α-DG compared with noninvasive human prostate cells (PWR-1E) and breast carcinoma (T47D) cells. α-DG protein and laminin-binding glycans were detected by 6C1 and VIA4–1 antibodies, respectively. (B) FACS analysis of expression of laminin-binding glycans (VIA4–1 and IIH6), α-DG core protein (6C1), CD44 and CD16, and α6- and β1-integrins of 2 subpopulations of PC3 cells. Laminin-111 binding to the cells was examined by immunofluorescence staining. (C) Tumor formation by subpopulations of PC3 cells in the orthotopic prostate. The weight of the prostate and regional lymph nodes and the tumor colony in the liver were examined 7 weeks after the inoculation of PC3 cells into the prostate of SCID mice. Representative primary tumors are shown on the left. (Scale bar: 1 cm.)