Fig. 2.

Immunolocalization of OsMAP65-3 in developing phragmoplasts. OsMAP65-3 was detected by an isoform specific antibody, MTs by an anti-α-tubulin antibody, and DNA by DAPI (4′,6-diamidino-2-phenylindole). Cells at an early and late stage of cytokinesis are shown. OsMAP65-3 appeared in a broader distribution pattern in the early phragmoplast, and then became more concentrated in the phragmoplast midline in the late phragmoplast. In the merged image, OsMAP65-3 was pseudocoloured in green, MTs in red and DNA in blue.