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. 2000 Aug 29;97(19):10619–10624. doi: 10.1073/pnas.180306597

Figure 1.

Figure 1

SREBP-1a activates the FAS promoter in vivo. (A) Northern blot analysis of both endogenous SREBP and SREBP transgene in −2100-FAS-CAT transgenic mice or −2100-FAS-CAT/SREBP-1a transgenic animals. Transgenic mice were either fasted for 24 h or fasted for 24 h then refed a high-carbohydrate/fat-free diet for 16 h. (B) mRNA levels for the endogenous FAS gene and CAT transgene analyzed by RNase protection assays using total liver RNA as described in Materials and Methods in transgenic mice carrying the −2100-FAS-CAT, the SREBP transgene, or both. Actin mRNA was used as a control. Essentially the same results were obtained from three independent experiments. F and R indicate fasted or refed states, respectively.