Figure 2.

Regulation by fasting/refeeding of CAT mRNA expression driven by the various FAS promoter regions. Total RNA isolated from liver was subjected to RNase protection assay to determine CAT, FAS, and actin mRNA levels as described in Materials and Methods and exposed to x-ray film (Fuji) for 1 day. * represents an autoradiogram on BioMax film (Kodak) exposed for 3 days, and F and R indicate fasted and refed states, respectively. Essentially the same results were obtained from three independent experiments.