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. 2000 Aug 29;97(19):10619–10624. doi: 10.1073/pnas.180306597

Figure 3.

Figure 3

Localization of the FAS promoter region mediating FAS transactivation by SREBP-1a. (A) Five micrograms of the indicated 5′ deletion FAS promoter-LUC constructs was cotransfected with 25 ng of an expression vector for SREBP-1a into 3T3-L1 fibroblasts. (B) Five micrograms of either −2100 FAS-LUC, −2100(−65) FAS-LUC, −444(−65)FAS-LUC, −184(−65) FAS-LUC, or −136(−65) FAS-LUC plasmids was cotransfected with 25 ng of an expression vector for SREBP-1a into 3T3-L1 fibroblasts. The values represent the mean ± standard deviation. Essentially the same results were obtained from two independent experiments.