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. Author manuscript; available in PMC: 2009 Aug 12.
Published in final edited form as: Neuron. 2009 Feb 12;61(3):397–411. doi: 10.1016/j.neuron.2008.12.024

Figure 5. Newly Exocytosed SypHluorins were Preferentially Retrieved in Both Endocytosis.

Figure 5

(A) Estimation of the amount of SypHluorins on the bouton surface at rest. A pulse of an acidic solution (pH 5.6) was applied before the single vesicle fusion being evoked at 0s. Averaging over 47 responses shows that the amount of SypHluorins on surface is about 93% of that inside a single vesicle.

(B) Example traces of single vesicle response before (left panel) and after photobleaching (right panel).

(C) Comparison of averaged fluorescence recovery after single vesicle fusion before (filled circle, n = 92 traces) and after photobleaching (open circle, n = 96 traces).

(D) Individual traces in C were realigned with the initial time of the fast endocytosis (left panel) or slow endocytosis (right panel), then averaged.

(E) Photobleaching surface SypHluorins reduces fluorescence intensity change in both the fast and slow endocytosis (See text). ***p < 0.001, paired t test.

Error bars represent SEM