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. Author manuscript; available in PMC: 2009 Aug 12.
Published in final edited form as: Neuron. 2009 Feb 12;61(3):397–411. doi: 10.1016/j.neuron.2008.12.024

Table 1.

Comparison of Sensor Performance in Cultured Hippocampal Neurons

F0 ΔF ΔF/F0 ΔF/F0½
Synaptophluorin 1 ± 0.04 1 ± 0.06 1 ± 0.05 1 ± 0.04
SypHluorin 1x 0.63 ± 0.02 1.21 ± 0.04 1.90 ± 0.10 1.52 ± 0.07
SypHluorin 2x 0.47 ± 0.02 2.01 ± 0.06 4.28 ± 0.20 2.93 ± 0.10
SypHluorin 4x 0.31 ± 0.02 1.74 ± 0.05 5.57 ± 0.30 3.12 ± 0.14

F0: fluorescence intensity of synapses before stimulation

ΔF: the peak change in fluorescence intensity upon stimulation

ΔF/F0: the signal-background ratio

ΔF/F01/2: the signal-noise ratio

Data are obtained from a stimulation with 40 action potentials at 20Hz, normalized to the corresponding value of Synaptophluorin, presented as mean ± SEM