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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Nat Med. 2009 Jun 14;15(7):808–813. doi: 10.1038/nm.1982

Figure 2. Wnt signaling inhibits CD8+ T cell proliferation and effector differentiation.

Figure 2

a–c,CFSE-labeled naive pmel-1 CD8+ T cells were primed in vitro with CD8+ T cell depleted splenocytes pulsed with 1 μM hgp10025–33, in conjunction with 10 ng ml−1 IL-2 and titrated doses of TWS119. Four days following T cell activation, phenotypic (a) CFSE dilution assays (b) and cytokine and 51Cr release assays (c) were performed. Data in panel c are represented as mean +/− SEM. d, Quantitative RT-PCR analysis of the expression of Eomes in CD8+ T cells after priming with anti-CD3 and anti-CD28 specific antibodies with or without 7 μM TWS119. Data are represented as mean +/−SEM. e, Flow cytometry and enumeration of T cell subsets six days after vaccination with or without TWS119. WT mice received adoptive transfer of 106 naive pmel-1 thy1.1+ CD8+ T cells in conjunction with recombinant fowlpox-based hgp100 vaccine. Mice received four daily doses of TWS119 (at 30 mg kg−1) from day 0 to day 3 or DMSO as control. All data are representative of at least two independently performed experiments.