Table 3.

Cellular concentration of insulin receptor and IRS1.^a

	Diet, % of baseline	T2D, % of controls
Insulin receptor	57 ± 7, P = 0.001	55 ± 12, P = 0.035
IRS1	103 ± 21	98 ± 30

^aThe amounts of insulin receptor or IRS1 in adipocytes were compared at baseline and after 4 wks on the high-calorie diet. Samples from the same subjects were analyzed on the same gel by SDS-PAGE and immunoblotting. Results are expressed as percentage of the amount of protein at the start (baseline) of the high-energy diet, mean ± SE (n = 6). The amounts of insulin receptor or IRS1 were also compared in adipocytes from subjects with T2D (age 33–72 years, average 53 years; BMI 28–48 kg/m², average 36 kg/m² [n = 16]), with cells from nondiabetic controls (age 34–80 years, average 62 years; BMI 20–39 kg/m², average 28 kg/m² [n = 17]). Samples from subjects with and without T2D were analyzed on the same gels, by SDS-PAGE and immunoblotting. Results are expressed as a percentage of nondiabetic controls, mean ± SE. P, paired Student t test.