Table 2. Functional overview of significant categorized MSigDB gene sets based on the pathways altered by both wild-type and T. b. rhodesiense inhibited for brucipain activity.

	Cytokines	Transcription Factors	Cell Surface Markers	Kinases	Translocated Genes	Oncogenes	Tumor Suppressors
Tumor suppressors		TP53					CDKN2A, CFL1, PTEN, TP53
Oncogenes				CDK4, RAF1	CCND1	CCND1, CDK4, HRAS, MDM2, PTPN1, RAF1
Translocated genes		NFKB2	TRFC	LCK	CCND1, LCK, NFKB2, TRFC
Kinases				ACTR2, CAMK2B, CDK2, CDK4, FYN, LCK, MAP2K2, MAP3K3P, PAK4, RAF1, RPS6KA1, RPS6KA2, RPS6KB2
Cell surface Markers			CD38, TRFC
Transcription factors		ERCC3, GATA3, GTF2F 1, HDAC1, HMGB1, KLF5, MEF2A, MEF2B, MYOD1, NFATC3, NFKB2, NFKBIB, NFKBIE, NFYB, NROB, NR1H3, RELA, STAT1, TAF6, TAF9, TP53
Cytokines	IFNGR1, IFNGR2, IL2RG, IL6

A functional overview of the MSigDB gene sets categorized into a small number of selected gene families whose members a common feature such as homology or biochemical activity. They do not necessarily have common origins. Annotation of pathway genes significantly expressed in HBMEC only in response to T. b. rhodesiense inhibited for brucipain activity are shown in bold font.