Figure 5. Internalisation of Mcf1 is required for freezing.

(A) Stills from a timelapse movie (see Video S8) of hemocytes expressing shibire^ts1 following injection with 0.2 µg µl⁻¹ Mcf1. Unlike wildtype cells, shibire mutant hemocytes do not freeze after exposure to Mcf1 and continue to migrate (asterisk) and extend large actin rich protrusions (arrows). Elapsed time is indicated in the upper right corner. (B) Injection of Alexa Fluro 555 labelled Mcf1 (Mcf1 555) into wildtype embryos shows localisation of labelled Mcf1 in hemocytes (white arrows) 1 hour after injection. Mcf1 can also be seen outside hemocytes indicating localisation in other surrounding cell types (arrowheads). Scale bars represent 10 µm.