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. 2009 Jul 17;4(7):e6267. doi: 10.1371/journal.pone.0006267

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Rochais et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) E10.5 wholemount embryos (left) and cryosections after X-gal staining (top) or in situ hybridization with a lacZ (T55 embryo, middle) or Hes1 riboprobe (bottom). The transgene is expressed at a subset of sites of Hes1 expression including the forelimb (fl), pharyngeal region (including ectoderm, arrowheads), neural tube (nt), including a specific population of neurons in the ventral neural tube (arrows) and diencephalon (d); ph, pharynx. Asterisk indicates probe trapping in the otic vesicle. (B) E12.5 forelimb (left) and lung (right) after X-gal staining (top) and nlacZ (middle) or Hes1 (bottom) in situ hybridization, showing similar transgene and Hes1 expression in the interdigital region (arrows) and pulmonary epithelium (arrowheads). Scale bar: 200 µm.