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(i) Interactions between the FIS2, MEA, FIE, and EZA1 proteins in the yeast two-hybrid system. The plates show growth of yeast containing different plasmid combinations on SC minus tryptophan, leucine, and histidine media containing 1 mM 3-aminotriazole. The label “empty” corresponds to either the bait vector pGBT9 or the prey vector pGAD424 without insert. (ii) Schematic drawing showing MEA proteins of different lengths used in the two-hybrid system.
