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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Int J Cancer. 2009 May 15;124(10):2450–2459. doi: 10.1002/ijc.24195

Figure 6. Kinetic effect on proteasome inhibition and cell death induction by shikonin in P388 leukimia cells.

Figure 6

P388 cells were treated with 2 μmol/L of shikonin for different hours, followed by measurement of the protesomal CT-like activity (A) and Western blotting analysis using specific antibodies against ubiquitinated proteins (Ub-prs), PARP, GAPDH (loading control, B) and annexin-PI binding assay (C). Columns, means of independent triplicate experiments; bars, SD. Cell death rate (%) was shown.