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. Author manuscript; available in PMC: 2009 Jul 9.
Published in final edited form as: Nature. 2008 Mar 20;452(7185):365–369. doi: 10.1038/nature06641

Figure 4. Expression of a stable REST mutant or oncogenic REST-FS leads to chromosomal instability.

Figure 4

a, Left: percentage of aberrant anaphases in HCT116 cells infected as in Fig. 3b. Right: representative pictures. Arrows point to lagging chromosomes and chromosome bridges. b, Premature sister-chromatid separation in cells expressing stable REST. Panels show representative metaphase spreads in HCT116 cells infected as in Fig. 3b. c, Nocodazole was added for the indicated durations to HCT116 cells infected as in Fig. 3b. Cells were stained with an anti-phospho-HH3 (pHH3) antibody to quantify their mitotic index. Where present, error bars represent s.d. (n = 3).