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. Author manuscript; available in PMC: 2009 Jul 9.
Published in final edited form as: Exp Eye Res. 2008 Jan 3;86(4):576–585. doi: 10.1016/j.exer.2007.12.007

Fig. 1.

Fig. 1

Immunostaining for ΔNp63 in normal human tissue. (A–D) Representative XY slices of whole mount human corneal epithelium double-labeled with a rabbit polyclonal antibody directed against the N terminus of ΔNp63 (green) and phalloidin (red). (A) Basal cell layer; (B) wing cell layer; (C) superficial cell layer; (D) negative control, primary antibody omitted. (E, F) 10 µm cryosections double-labeled with anti-rabbit ΔNp63 (green) and PI (red). (E) Human central corneal epithelium. Arrows indicate DNp63-negative, PI-positive cells; (F) negative control, primary antibody omitted. Whole mount experiments were repeated three independent times; cryostat sectioned experiments were repeated three independent times. Scale: 50 µm.