Figure 1.

Micrograph from a typical Ca²⁺ flux measurement during maize IVF. The Ca²⁺-selective vibrating probe was positioned approximately 1 μm from the surface of the egg cell at its nearest vibration point. The probe oscillated with an excursion of 10 μm away from the cell (Osc.). The angle between the probe and the adhering male gamete is indicated by the black arrow.