Figure 4.

Influence of the position of the probe, respective to that of the adhering male gamete, on the measurement of the Ca²⁺ influx induced by fusion. Lag time between fusion and onset of Ca²⁺ influx is plotted against the angle between the male gamete and the Ca²⁺-selective electrode. The drawing (Lower) illustrates the different positions estimated visually during the adhesion procedure. Only differences of at least 45° have been taken into account.