Figure 2. Effects of butyrate on the expression of the γ-, β- and α-globin genes.
Mononuclear cells from patients with β-Thal (×,+, ◇, □, ▵, ○, ●, ▲) and SCD (*, ×, +, ▵, ○) were cultured into BFU-E derived colonies in absence (control) or presence of 150μM butyrate. mRNA levels were measured by quantitative real-time RT-PCR and expressed as fold change relative to the control, % γ/(β+ γ) and ratio α/(β+ γ). Solid horizontal lines represent the mean value for every condition. In each experiment, each sample had triplicates $: p≤0.05