Table 1.
Quantitative PCR validation.
Age | Gene | TG ± SEM* | WT ± SEM* | QPCR
TG/WT |
Array
TG/WT |
---|---|---|---|---|---|
3 month | Cyclin D2 | 0.179±0.016 | 0.222±0.031 | 0.806 | 0.556 |
Tiparp | 0.255±0.011 | 0.363±0.027 | 0.702 | 0.509 | |
9 month | Cyclin D2 | 0.180±0.017 | 0.363±0.072 | 0.496 | 0.444 |
Tiparp | 0.471±0.088 | 0.844±0.105 | 0.558 | 0.493 |
Comparison of microarray results with QPCR results for two genes, cyclin D2 and TCDD-inducible poly(ADP-ribose) polymerase (Tiparp), which were altered at both three and nine months.
QPCR results shown in table were normalized to pyruvate decarboxylase.