Table 1.
Enrichment of DGDG in the extraplastidic membrane fraction
| Plant | Pi | Fraction | DGDG | MGDG | PE | D/M | D/P |
|---|---|---|---|---|---|---|---|
| WT | + | Pellet | 19.6 | 52.2 | 1.6 | 0.38 | 12.25 |
| + | SN | 4.5 | 13.5 | 26.3 | 0.34 | 0.17 | |
| − | Pellet | 28.0 | 50.4 | 0.9 | 0.56 | 31.11 | |
| − | SN | 31.1 | 16.8 | 11.2 | 1.85 | 2.78 | |
| dgd1 | + | Pellet | 1.9 | 52.4 | 2.2 | 0.04 | 0.86 |
| + | SN | 1.3 | 12.8 | 29.0 | 0.10 | 0.04 | |
| − | Pellet | 6.1 | 52.4 | 2.5 | 0.12 | 2.44 | |
| − | SN | 18.1 | 10.5 | 19.0 | 1.72 | 0.95 |
Lipids were isolated from different subcellular fractions of wild type and dgd1 mutant grown in medium with 1 mM or no added phosphate (Pi). The mean of three independent lipid measurements is given in mol%, and standard errors were <15% for values >10 or <30% for values <10. D/M, ratio of DGDG/MGDG; D/P, ratio of DGDG/PE; SN, supernatant; WT, wild type.