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. 1983 Aug;18(2):287–291. doi: 10.1128/jcm.18.2.287-291.1983

Determination of herpes simplex virus type-specific antibodies by enzyme-linked immunosorbent assay.

R M Coleman, L Pereira, P D Bailey, D Dondero, C Wickliffe, A J Nahmias
PMCID: PMC270792  PMID: 6311871

Abstract

We determined type-specific antibodies to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) by an indirect enzyme-linked immunosorbent assay, using as antigens HSV-1 glycoprotein gC-1 and a HSV-2-specific polypeptide purified on affinity columns of monoclonal antibodies. All sera were initially screened for HSV antibodies by the enzyme-linked immunosorbent assay with a pool of Triton X-100-extracted antigens of HSV-1- and HSV-2-infected HEp-2 cells. The titer of HSV antibodies was predicted from a linear regression curve based on the absorbance of the initial 1:50 serum dilution. The sensitivity and specificity of the screening assay and of the assay for type-specific antibodies were established.

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Selected References

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  1. Arvin A. M., Koropchak C. M., Yeager A. S., Pereira L. Detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein C purified with monoclonal antibody. Infect Immun. 1983 Apr;40(1):184–189. doi: 10.1128/iai.40.1.184-189.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Back A. F., Schmidt N. J. Typing Herpesvirus hominis antibodies and isolates by inhibition of the indirect hemagglutination reaction. Appl Microbiol. 1974 Sep;28(3):400–405. doi: 10.1128/am.28.3.400-405.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baer G. M., Shaddock J. H., Moore S. A., Yager P. A., Baron S. S., Levy H. B. Successful prophylaxis against rabies in mice and Rhesus monkeys: the interferon system and vaccine. J Infect Dis. 1977 Aug;136(2):286–291. doi: 10.1093/infdis/136.2.286. [DOI] [PubMed] [Google Scholar]
  4. Bernstein M. T., Stewart J. A. Method for typing antisera to Herpesvirus hominis by indirect hemagglutination inhibition. Appl Microbiol. 1971 Apr;21(4):680–684. doi: 10.1128/am.21.4.680-684.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cremer N. E., Cossen C. K., Hanson C. V., Shell G. R. Evaluation and reporting of enzyme immunoassay determinations of antibody to herpes simplex virus in sera and cerebrospinal fluid. J Clin Microbiol. 1982 May;15(5):815–823. doi: 10.1128/jcm.15.5.815-823.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Denoyel G. A., Gaspar A., Nouyrigat C. Enzyme immunoassay for measurement of antibodies to herpes simplex virus infection: comparison with complement fixation, immunofluorescent-antibody, and neutralization techniques. J Clin Microbiol. 1980 Feb;11(2):114–119. doi: 10.1128/jcm.11.2.114-119.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dreesman G. R., Matson D. O., Courtney R. J., Adam E., Melnick J. L. Detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay. Intervirology. 1979;12(2):115–119. doi: 10.1159/000149077. [DOI] [PubMed] [Google Scholar]
  8. Eberle R., Courtney R. J. Assay of type-specific and type-common antibodies to herpes simplex virus types 1 and 2 in human sera. Infect Immun. 1981 Mar;31(3):1062–1070. doi: 10.1128/iai.31.3.1062-1070.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eisenberg R. J., Ponce de Leon M., Pereira L., Long D., Cohen G. H. Purification of glycoprotein gD of herpes simplex virus types 1 and 2 by use of monoclonal antibody. J Virol. 1982 Mar;41(3):1099–1104. doi: 10.1128/jvi.41.3.1099-1104.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Forghani B., Schmidt N. J., Lennette E. H. Solid phase radioimmunoassay for typing herpes simplex viral antibodies in human sera. J Clin Microbiol. 1975 Nov;2(5):410–418. doi: 10.1128/jcm.2.5.410-418.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nahmias A. J., Dowdle W. R. Antigenic and biologic differences in herpesvirus hominis. Prog Med Virol. 1968;10:110–159. [PubMed] [Google Scholar]
  12. Nahmias A. J., Dowdle W. R., Kramer J. H., Luce C. F., Mansour S. C. Antibodies to Herpesvirus hominis types 1 and 2 in the rabbit. J Immunol. 1969 Apr;102(4):956–962. [PubMed] [Google Scholar]
  13. Nahmias A. J., Josey W. E., Naib Z. M., Luce C. F., Duffey A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. I. Patients with genital herpetic infections. Am J Epidemiol. 1970 Jun;91(6):539–546. doi: 10.1093/oxfordjournals.aje.a121165. [DOI] [PubMed] [Google Scholar]
  14. Nahmias A., DelBuono I., Pipkin J., Hutton R., Wickliffe C. Rapid identification and typing of herpes simplex virus types 1 and 2 by a direct immunofluorescence technique. Appl Microbiol. 1971 Sep;22(3):455–458. doi: 10.1128/am.22.3.455-458.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Patterson W. R., Rawls W. E., Smith K. O. Differentiation of serum antibodies to herpesvirus types 1 and 2 by radioimmunoassay. Proc Soc Exp Biol Med. 1978 Feb;157(2):273–277. doi: 10.3181/00379727-157-40036. [DOI] [PubMed] [Google Scholar]
  16. Pauls F. P., Dowdle W. R. A serologic study of herpesvirus hominis strains by microneutralization tests. J Immunol. 1967 May;98(5):941–947. [PubMed] [Google Scholar]
  17. Pereira L., Dondero D. V., Gallo D., Devlin V., Woodie J. D. Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies. Infect Immun. 1982 Jan;35(1):363–367. doi: 10.1128/iai.35.1.363-367.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pereira L., Klassen T., Baringer J. R. Type-common and type-specific monoclonal antibody to herpes simplex virus type 1. Infect Immun. 1980 Aug;29(2):724–732. doi: 10.1128/iai.29.2.724-732.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Plummer G. A review of the identification and titration of antibodies to herpes simplex viruses type 1 and type 2 in human sera. Cancer Res. 1973 Jun;33(6):1469–1476. [PubMed] [Google Scholar]
  20. Rawls W. E., Iwamoto K., Adam E., Melnick J. L. Measurement of antibodies to herpesvirus types 1 and 2 in human sera. J Immunol. 1970 Mar;104(3):599–606. [PubMed] [Google Scholar]
  21. Seth P., Prakash S. S., Ghosh D. Antibodies to Herpes simplex virus types 1 and 2 in patients with squamous-cell carcinoma of uterine cervix in India. Int J Cancer. 1978 Dec;22(6):708–714. doi: 10.1002/ijc.2910220612. [DOI] [PubMed] [Google Scholar]
  22. Shore S. L., Black C. M., Melewicz F. M., Wood P. A., Nahmias A. J. Antibody-dependent cell-mediated cytotoxicity to target cells infected with type 1 and type 2 herpes simplex virus. J Immunol. 1976 Jan;116(1):194–201. [PubMed] [Google Scholar]
  23. Smith J. W., Glorioso J. C. Effect of cross-immunization on monotypic antibody responses to herpes simplex virus types 1 and 2. J Immunol. 1976 Apr;116(4):898–903. [PubMed] [Google Scholar]
  24. Smith J. W., Lowry S. P., Melnick J. L., Rawls W. E. Antibodies to surface antigens of herpesvirus type 1- and type 2-infected cells among women with cervical cancer and control women. Infect Immun. 1972 Mar;5(3):305–310. doi: 10.1128/iai.5.3.305-310.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vestergaard B. F., Grauballe P. C. ELISA for herpes simplex virus (HSV) type-specific antibodies in human sera using HSV type 1 and type 2 polyspecific antigens blocked with type-heterologous rabbit antibodies. Acta Pathol Microbiol Scand B. 1979 Aug;87(4):261–263. doi: 10.1111/j.1699-0463.1979.tb02436.x. [DOI] [PubMed] [Google Scholar]
  26. Vestergaard B. F., Grauballe P. C., Spanggaard H. Titration of herpes simplex virus antibodies in human sera by the enzyme-linked immunosorbent assay (ELISA). Acta Pathol Microbiol Scand B. 1977 Dec;85B(6):466–468. doi: 10.1111/j.1699-0463.1977.tb02004.x. [DOI] [PubMed] [Google Scholar]

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