Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2009 Jul 9.

Published in final edited form as: Chem Biol. 2007 Aug;14(8):944–954. doi: 10.1016/j.chembiol.2007.07.013

(A). Multiple sequence alignment of the helical N-terminal docking domains from the pikromycin and DEBS PKS. PikAIV(ΔDock) is the result of truncating the first 14 amino acids (in italics) of PikAIV. (B). Multiple sequence alignment of partial C-terminal docking domains from the pikromycin and DEBS PKS. PikAIII(ΔDock) is the result of truncating the final 19 amino acids (in italics) of PikAIII that comprise helix 3. (C). Radio-TLC of reaction products produced by the truncated pikromcyin modules. PikAIII(ΔDock) or PikAIV(ΔDock) was paired with their respective partner wild-type or truncated module in the presence of the synthetic pentaketide NAC thioester and 2-[¹⁴C]-methylmalonyl-CoA as described in the experimental procedures. Likewise, PikAIII or PikAIII(ΔDock) was paired with recombinant pikromycin TE under identical experimental condtions. The reaction products were resolved by TLC (5% MeOH in CHCl₃) and subsequently visualized using a Typhoon phosphorimager.

(A). Multiple sequence alignment of the helical N-terminal docking domains from the pikromycin and DEBS PKS. PikAIV(ΔDock) is the result of truncating the first 14 amino acids (in italics) of PikAIV. (B). Multiple sequence alignment of partial C-terminal docking domains from the pikromycin and DEBS PKS. PikAIII(ΔDock) is the result of truncating the final 19 amino acids (in italics) of PikAIII that comprise helix 3. (C). Radio-TLC of reaction products produced by the truncated pikromcyin modules. PikAIII(ΔDock) or PikAIV(ΔDock) was paired with their respective partner wild-type or truncated module in the presence of the synthetic pentaketide NAC thioester and 2-[¹⁴C]-methylmalonyl-CoA as described in the experimental procedures. Likewise, PikAIII or PikAIII(ΔDock) was paired with recombinant pikromycin TE under identical experimental condtions. The reaction products were resolved by TLC (5% MeOH in CHCl₃) and subsequently visualized using a Typhoon phosphorimager.