Figure 6. ACPdock Competition with PikAIII for Interaction with PikAIV.
Reaction mixtures containing wild type PikAIII and PikAIV were titrated with increasing amounts of the C-terminal docking domain of PikAIII (ACPdock) as described in the experimental procedures. The reaction products were resolved by TLC (5% MeOH in CHCl3) and subsequently visualized using a Typhoon phosphorimager. (A) Radio-TLC of macrolactone production versus increased ACPdock concentration; (B) Plot of relative macrolactone production versus ACPdock concentration.