Table 2.
Steady-State Kinetic Parameters for 10-Deoxymethynolide Production by PikAIII(ΔDock) and PikAIV(ΔDock)
Modules | kcat (min−1) | KM (mM) | kcat/KM (min−1·mM−1) | Relative kcat | Relative kcat/KM |
---|---|---|---|---|---|
PikAIII + PikAIV | 2.0 ± 0.02 | 0.31 ± 0.08 | 6.5 ± 1.7 | 1 | 1 |
PikAIII(ΔDock) + PikAIV | 0.044 ± 0.003 | 0.095 ± 0.02 | 0.46 ± 0.10 | 0.02 | 0.07 |
PikAIII + PikAIV(ΔDock) | 0.053 ± 0.002 | 0.064 ± 0.01 | 0.83 ± 0.13 | 0.03 | 0.13 |
PikAIII(ΔDock)+ PikAIV(ΔDock) | 0.051 ± 0.004 | 0.093 ± 0.03 | 0.55 ± 0.18 | 0.03 | 0.08 |
PikAIII + Pik Thioesterase | 0.12 ± 0.01 | 0.22 ± 0.06 | 0.55± 0.21 | 0.06 | 0.08 |
PikAIII(ΔDock) + Pik Thioesterase | 0.16 ± 0.002 | 0.08 ± 0.003 | 2 ± 0.8 | 0.08 | 0.3 |