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. Author manuscript; available in PMC: 2009 Jul 9.
Published in final edited form as: Immunity. 2007 Jul 12;27(1):111–122. doi: 10.1016/j.immuni.2007.05.016

Figure 4. Proliferation of CD28/CTLA-4-/- T cells is reduced by B7-1.

Figure 4

CD28/CTLA4-/- or WT CD4 T cells were stimulated with beads coated with anti-CD3 plus either B7-1-Ig (“B7-1”) or hIgG1Fc (“Ig”) control.

A. T cell proliferation was measured by 3H-thymidine incorporation for the last 16 hours of a 64 hour culture. These results are representative of at least 5 independent experiments.

B. T cell expansion was measured by flow cytometric analysis of CFSE dilution at 64 hours. These results are representative of 3 independent experiments.

C. Cytokines were measured by BD Cytokine Bead Array in supernatants obtained at 48 hrs.