Figure 5. Markers of activation are diminished in CD28/CTLA-4-/- T cells stimulated with anti-CD3 plus B7-1.
CD28/CTLA4-/- CD4 T cells were cultured with beads coated with anti-CD3 plus either B7-1-Ig (“B7-1”) or hIgG1Fc (“Ig”). T cells were then stained and analyzed by FACS after 24 or 48 hours for A. activation markers and B. costimulatory receptors and ligands. Shaded histogram indicates control cells.