Fig. 3.

Silencing of ORP10 enhances cholesterol and triglyceride biosynthesis in Huh7 cells. a Western blot analysis of ORP10 protein level in cells transfected for 48 h with nontargeting control (siNT) or ORP10-specific siRNAs (siORP10.1, siORP10.2) using INTERFERin. Anti-β-actin was used as a control for equal protein loading. The apparent molecular mass of the protein detected by the antibody (85 kDa) matches the ORP10 mass deduced from the cDNA sequence (83.97 kDa). b Incorporation of [³H]acetate into free cholesterol (FC), cholesteryl esters (CE), and triglycerides (TG) in cells treated with control or ORP10-specific siRNAs (N = 4). c HMG-CoA reductase activity (expressed as [¹⁴C]HMG-CoA incorporation into mevalonic acid, DMP per milligrams membrane protein) in cells treated with control or ORP10-specific siRNAs (N = 4). d Incorporation of [³H]oleic acid into triglycerides (TG) in cells treated with control or ORP10-specific siRNAs (N = 4). The results represent mean ± s.e.m. Statistical significance of the differences between values obtained for siNT and the ORP10-specific siRNAs was evaluated using Student’s t test; *p < 0.05, **p < 0.01