Fig. 4.

Silencing of ORP10 increases the accumulation of apoB100 in the growth medium. Accumulation of apoA-I (a) and apoB100 (b) into the growth medium of Huh7 cells treated with nontargeting (siNT) or ORP10-specific (siORP10.1, siORP10.2) siRNAs during a 24-h time course was quantified with specific sandwich ELISA assays. The data represents a mean ± s.e.m. (aN = 3, bN = 5; *p < 0.05, **p < 0.01, t test). c Analysis of apoB100 accumulation into growth medium at the indicated time points by Western blotting. An experiment representative of three independent analyses with similar results is shown