Fig. 5.

OSBPL10 silencing has no significant effect on the mRNAs for key factors involved in lipid metabolism. Huh7 cells were transfected for 48 h with control (siNT) or ORP10-specific siRNAs (siORP10.1, black bars; siORP10.2, gray bars), followed by total RNA isolation and quantitative RT-PCR analysis of mRNA levels. SREBP sterol regulatory element binding protein, LXR liver X receptor, HMGCoR 3-hydroxy-3-methylglutaryl coenzyme A reductase, LDLR low-density lipoprotein receptor, ABC ATP-binding cassette transporter, Insig insulin-induced gene, FAS fatty acyl synthase, SCD stearoyl coenzyme A desaturase-1. The data depict mRNA levels in ORP10 siRNA-treated cells relative to those in cells transfected with the scrambled control siRNA (siNT) and represents a mean ± s.e.m. (N = 4–5)