Fig. 4.

Fluorescence microscopy of E. coli (a, b, c, d) and P. aeruginosa (e, f, g, h), treated with either polymyxin B, miconazole or with both substances in combination for 18 h. Cells were then stained with SYTO9 and PI. Identical pictures of bacterial suspensions are generated with a green (left pictures) or red (right pictures) filter set. The red stained cells represent bacteria with damaged membranes and the green cells denote those with intact membranes. Untreated bacterial cells (a, e), bacteria treated with 0.73 μg/ml miconazol (b, f), with 0.73 μg/ml polymyxin B (c, g) and with 0.18 μg/ml miconazole plus 0.18 μg/ml polymyxin B (d, h)