Fig. 2.

Chromatin immunoprecipitation. a Formalin-fixed and enzymatically sheared chromatin from five healthy individuals heterozygous at rs2569190 was immunoprecipitated with antibodies directed against RNA polymerase II as described in the “Materials and methods” section or was left untreated as an “input” control. Purified input and output DNA samples were subjected to amplification spanning rs2569190. Products of 201 bp in size were subjected to restriction enzyme digestion with HaeIII. Restriction digest analysis was carried out in a bioanalyzer yielding molar amounts of each fragment as given. Undigested fragments represent DNA of the T allele. Fragments of 49 and 152 bp in size represent DNA of the C allele. Material from homozygous individuals (TT and CC) is shown as a control for complete and specific enzymatic activity. b Haplotypes (rs5744455–rs2569190–rs4914–rs2563298) of the five donors are given