Effect of hyoscine on spontaneous contractile activity in the whole guinea pig bladder and isolated muscle strips in vitro. (A and B) Application of hyoscine (3 µmol·L−1) in the presence of tetrodotoxin (TTX) (1 µmol·L−1) inhibited spontaneous contractions of the whole bladder in vitro (A) and of muscle strips (B). (C and D) Averaged data of the effects of hyoscine (3 µmol·L−1) in the presence of TTX (1 µmol·L−1) on spontaneous contractions of the whole bladder (C, N= 4) and of isolated muscle strips (D, n= 8, N= 7). * and #, significant difference from TTX, P < 0.01 and P < 0.05 respectively.