Figure 2.

Effect of hyoscine and tetrodotoxin (TTX) on distension-induced contractile activity in the whole guinea pig bladder, in vitro. (A) Typical recording of 4th distension (0.5 mL·min⁻¹ for 6 min)-evoked contractile activity and (B) 5th distension after 15 min of application of hyoscine (3 µmol·L⁻¹). (C) Decay of distension-evoked contractile activity in control experiments (N= 5). Note that 4th–6th distensions evoked similar contractile activity of the bladder. (D) Averaged data of the effects of hyoscine (3 µmol·L⁻¹, N= 5) and TTX (1 µmol·L⁻¹, N= 5) on 3 mL distension-induced bladder contractions. *, significant difference from control, P < 0.01.