Figure 7.
Effects of physostigmine and density of intramural neurones in the fresh and cultured muscle strips of the rat bladder. (A) Typical traces demonstrate the effects of physostigmine (3 µmol·L−1) on a fresh muscle strip of the rat bladder in the presence of tetrodotoxin (TTX) (1 µmol·L−1). Note that TTX blocked EFS-induced contractions (evoked by 10 Hz, marked by filled dots). (B) Averaged data on the density of intramural neurones (number of neurones divided by area of the tissue) in fresh and 5 day cultured muscle strips from the rat bladder. Note the very different vertical scale from Figure 5B. (C) Physostigmine (3 µmol·L−1)-induced contractile effect was significantly reduced after 5 days in organ culture compared with control fresh strips. Each value in (B) and (C) is the mean from four to five rats. *, significant difference from fresh tissue, P < 0.01.