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. 2009 Apr 9;157(3):404–414. doi: 10.1111/j.1476-5381.2009.00174.x

Figure 3.

Figure 3

Quantitative real-time RT-PCR experiments and expression of cardiac Na+ channel proteins (Nav1.5, Navβ1 and Navβ2) assessed by Western blots analysis. Bar graphs represent amount of Nav1.5 mRNA or β-subunits mRNA. (A and B) mRNA for Nav1.5 extracted from cardiomyocytes with or without 10 µmol·L−1 bepridil (bep) treatment for 24 h (A) and 48 h (B). (C) mRNA for Nav1.5 from human embryonic kidney (HEK)-Nav1.5 cells with or without 10 µmol·L−1 bepridil treatment for 24 h. (D) Comparison of four different β-subunits genes (SCN1B, SCN2B, SCN3B and SCN4B) level in cardiomyocytes treated with or without 10 µmol·L−1 bepridil for 24 h. Representative PCR products are shown in inset with the reference gene GAPDH (below). Each mRNA product was normalized to that of GAPDH. Numbers of experiment are indicated in parentheses. (E) Examples of Nav1.5, Navβ1, Navβ2 and actin protein expression in neonatal rat cardiomyocytes. (F) Protein expression levels of Nav1.5, Navβ1 and Navβ2 determined from the density of blotted bands in panel (E). Na+ channel proteins extracted from cardiomyocytes treated with vehicle (vehi), 10 µmol·L−1 bepridil and 20 µmol·L−1 W-7 for 24 h. **P < 0.01 compared with vehicle.