Figure 1.
Construction of a disruptant of slr1369 from Synechocystis sp. PCC6803. (a) Plasmid construction for disruption of slr1369 in Synechocystis sp. PCC6803. (b) Confirmation by PCR of the disruption of slr1369 by insertion of the kanamycin-resistance (Kmr) gene in SNC1. Sense and antisense primers, corresponding to the 5′- and 3′-terminal regions of slr1369, respectively, were used for the PCR with genomic DNA of the wild type or SNC1 as a template. (c) PG-requiring phenotype of SNC1. Wild-type and SNC1 cells were cultured on agar plates of BG-11 with or without PG supplementation.