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AUC experiments with Fc-FITC indicated PbA does not self-associate through Fc–Fc or Fc-peptide interactions. The sw of 0.3 mg/mL FITC-Fc obtained from SV absorbance (495 nm) experiments at 60,000 rpm matched those of the unlabeled Fc (Panel A); the c(s) distribution of FITC-Fc was unchanged by the addition of PbA to 0.3 mg/mL (Panel B).
