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(A) SMRC of 13C-labeled CMG2 (4 μM), either in the absence (red) or presence (blue) of PA (4 μM) at pH 8.0 (left spectra) or pH 5 (right spectra). (B) SMRC of 13C-labeled CMG2 (6.25 μM), either in the absence (red) or presence (blue) of prepore (3.1 μM PA63) at pH 8.0 (left panel) or pH 5 (right panel). Spectra represent 512 transients 20°C.
