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(A) Binding 2-FHis-CMG2 (40 μM) in the absence (blue) and presence (red) of PA (40 μM) monitored by 19F NMR. Spectra were acquired at 20°C, pH 8.0 and represent 4096 transients. (B) SMRC of 13C-2-FHis-CMG2 (12.5 μM), either in the absence (red) or presence (blue) of prepore (6.25 μM PA63), as a function of pH. Spectra represent 512 transients acquired at 20°C.
