Figure 2.

Thermal melts of the mutant proteins by circular dichroism spectroscopy. The concentrations of all proteins were set to equal concentration prior to the melts to a common optical density (OD_{280 nm} = 0.24). All thermal melts were carried out between 25 and 80°C. (The unique thermal transitions between 25 and 65°C are shown here.) As can be seen in the transition temperatures, all mutant proteins are significantly destabilized compared with the wild type by at least 6°C or more. GST50D is the most destabilized protein by ∼15°C.