Table III.

Split Sites Obtained in This Work and Previously Reported

	Group 1a	Group 2
(a) TSα
Sites obtained in this work	L40, I64, T68, A137, D161, I166, A180, T183, A185	G26, S215,
Previously reported resultsb	E42, A73, E135, R145, P155, G170, R188, P192,	N108, K120,
(b) TEM-1 beta-lactamase
Sites obtained in this work	Y46, I47, F60, N100, E177, T181, R191, A217,A232, S235	D38, L40, L113, A126, M155, G265
Previously reported sitesc	F66, G196, E197, L198, K215
Sites used in SCHEMA recombinationd	G41, R65, M69, Y105, S130, N132, T149, R161, D163, D176, D179, T189, L190, V216, G218, P226, S266	S70, K73, W165
(c) APH(3′)-I
Sites obtained in this work	A62, V65, D67, L81,	Y26, F87, T90, G117, D122, V126, A152, W173, D198, V217, R246, I253, F263,
Previously reported sitese	D64 (E59 in APH(3′)-IIa), T76-F78 (A71-G74 in APH(3′)-IIa)	G103(G99 in APH(3′)-IIa)

^aGroup 1: Sites within 10 residues of the previously reported sites; Group 2: sites identified differently in this work and other reports.

^bSites reported by Yamagishi and co-workers.¹⁰

^cSites reported previously.^9,24,28

^dSites reported by Arnold and co-workers.^4,29,30

^eThese sites were homologous to the breakage points reported for APH(3′)-IIa.^11,31