Table I.
Unfolding Kinetic Parameters of MBP, MBP•Maltose Complex, and RNase H Determined by Pulse Proteolysis and Circular Dichroism in Urea
| Protein | Method | ku(H2O) (s−1) | mu (kcal/(mol M)) |
|---|---|---|---|
| MBP | Pulse proteolysis | (6.0 ± 1.6) × 10−7 | 0.96 ± 0.04 |
| CD | (12.4 ± 1.1) × 10−7 | 0.87 ± 0.01 | |
| MBP + 100 μM maltose | Pulse proteolysis | (9.4 ± 3.2) × 10−9 | 1.01 ± 0.03 |
| RNase H | Pulse proteolysis | (2.1 ± 0.1) × 10−5 | 0.40 ± 0.03 |
| CDa | (1.69 ± 0.04) × 10−5 | 0.42 ± 0.03 |
From Ref.11.